Urinary analysis of four testosterone metabolites and pregnanediol by gas chromatography-combustion-isotope ratio mass spectrometry after oral administrations of testosterone

Titre du document / Document title

Auteur(s) / Author(s)

MAITRE Arnaud⁽¹⁾ ; SAUDAN Christophe⁽¹⁾ ; MANGIN Patrice⁽¹⁾ ; SAUGY Martial⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, SUISSE

Résumé / Abstract

The most frequently used method to demonstrate testosterone abuse is the determination of the testosterone and epitestosterone concentration ratio (T/E ratio) in urine. Nevertheless, it is known that factors other than testosterone administration may increase the T/E ratio. In the last years, the determination of the carbon isotope ratio has proven to be the most promising method to help discriminate between naturally elevated T/E ratios and those reflecting T use. In this paper, an excretion study following oral administration of 40 mg testosterone undecanoate initially and 13 h later is presented. Four testosterone metabolites (androsterone, etiocholanolone, 5α-androstanediol, and 5β-androstanediol) together with an endogenous reference (5β-pregnanediol) were extracted from the urines and the δ¹³C/¹²C ratio of each compound was analyzed by gas chromatography-combustion-isotope ratio mass spectrometry. The results show similar maximum δ¹³C-value variations (parts per thousand difference of δ¹³C/¹²C ratio from the isotope ratio standard) for the T metabolites and concomitant changes of the T/E ratios after administration of the first and the second dose of T. Whereas the T/E ratios as well as the androsterone, etiocholanolone and 5α-androstanediol δ¹³C-values returned to the baseline 15 h after the second T administration, a decrease of the 5β-androstanediol δ-values could be detected for over 40 h. This suggests that measurements of 5β-androstanediol δ-values allow the detection of a testosterone ingestion over a longer post-administration period than other T metabolites δ¹³C-values or than the usual T/E ratio approach.

Revue / Journal Title

Journal of analytical toxicology ISSN 0146-4760 CODEN JATOD3

Source / Source

Congrès
Joint Meeting of the Society of Forensic Toxicologists and the International Association of Forensic Toxicologists , ETATS-UNIS (28/08/2004)
2004, vol. 28, n^o 6 (150 p.) [Document : 6 p.] (17 ref.), pp. 426-431 [6 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Preston, Niles, IL, ETATS-UNIS (1977) (Revue)

Mots-clés anglais / English Keywords

Urine ; Combustion ; Isotopic composition ; Mass spectrometry ; Coupled method ; Gas chromatography ; Human ; Oral administration ; Pharmacokinetics ; Elimination ; Biological fluid ; Steroid ; Androgen ; Metabolite ; Testosterone ; Quantitative analysis ; Chemical analysis ;

Mots-clés français / French Keywords

Urine ; Combustion ; Composition isotopique ; Spectrométrie masse ; Méthode couplée ; Chromatographie phase gazeuse ; Homme ; Voie orale ; Pharmacocinétique ; Elimination ; Liquide biologique ; Stéroïde ; Androgène ; Métabolite ; Testostérone ; Analyse quantitative ; Analyse chimique ;

Mots-clés espagnols / Spanish Keywords

Orina ; Combustión ; Composición isotópica ; Espectrometría masa ; Método acoplado ; Cromatografía fase gaseosa ; Hombre ; Vía oral ; Farmacocinética ; Eliminación ; Líquido biológico ; Esteroide ; Andrógeno ; Metabolito ; Testosterona ; Análisis cuantitativo ; Análisis químico ;

Localisation / Location

INIST-CNRS, Cote INIST : 17415, 35400011403152.0070

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Nº notice refdoc (ud4) : 16096186